Practice Quiz for General Genetics
Chapter 8 -- Bacterial and Viral Genetic Systems

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1. Which of the following cells is a prototroph?
A. his+met+pro+
B. his-met+lys+
C. glu-try-lys-
D. thy+met-lys+

2. The bacterial genome exists as
A. several circular pieces of DNA with many proteins attached
B. several long strands of DNA
C. a single long strand of essentially naked DNA
D. a circular piece of essentially naked DNA

3. The process by which a bacterial cell picks up exogenous pieces of DNA directly from the media and incorporates it into its genome is called
A. conjugation
B. transformation
C. transduction
D. translocation

4. In a transformation experiment in which the a+b+ strain is the donor to the ab strain, the following transformant were recovered: a+b = 145, ab+ = 125, a+b+ = 210. The distance between the a locus and the b locus is
A. 33
B. 50
C. 25
D. too far apart to calculate

5. The process by which genetic material is transfered from one bacterial cell to another through a sex pilus is called
A. conjugation
B. transduction
C. translocation
D. transformation

6. In a transformation experiment in which the his+met+ strain was the donor, the following transformants were isolated: his-met+ = 34, his+met- = 28, his+met+ = 194. What is the map distance between these loci?
A. 76 m.u.
B. 24 m.u.
C. 32 m.u.
D. too far apart to map

7. The F factor that allows cells to be donors during conjugation is a
A. protein
B. mitochondria
C. plasmid
D. phage

8. In the conversion of an F+ strain to an Hfr strain, the F factor incorporates into the bacterial chromosome
A. before the leu locus
B. between the gal locus and the thy locus
C. at random with respect to location and orientation
D. oriented in a clockwise direction

9. Once an exogenous piece of DNA is in the bacterial cell, it is incorporated into the bacterial genome through
A. gene conversion
B. crossing-over
C. translocations
D. mutations

10. Several Hfr strains are mated with an auxotrophic F- strain by the interrupted mating technigue. The order of transfer for the loci are given in the table.
         strain 1     strain 2      strain 3    strain 4
           thr          str           his         thy
           lip          thy           thy         his
           trp          his           str         trp
           his          trp           ilv         lip
           thy          lip           thr         thr
What is the order of the loci on the chromosome?
A. str-thy-his-trp-lip-ilv-thr
B. thr-lip-trp-his-thy-ilv-str
C. thy-his-trp-lip-thr-thy-his
D. thr-lip-trp-his-thy-str-ilv

Last updated on 24 October 2004.
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